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Class 35 - Gene mapping in humans Linkage mapping Steps in...

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Gene mapping in humans
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Linkage mapping Steps in human recombination mapping Examine progeny of a crosses in families Determine those that are recombinant (R) and those that are non-recombinant (NR) Based on observed R and NR in each family estimate the most probable rate of recombination as an estimate of linkage 2
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Reasons human mapping is “different” Cannot do test crosses in humans Can’t always determine unambiguously if progeny are recombinant Small families so number of recombinants low Can be hard to find Must rely on statistics of small numbers 3
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First problem - which child is a recombinant in a family Must determine, from pedigrees, who is recombinant The biggest problem is determining phase in the parents 4
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First problem - which child is a recombinant in a family Must determine, from pedigrees, who is recombinant The biggest problem is determining phase in the parents 4 D A d a D a d A D AND A in cis D AND A in trans
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So in this pedigree it is pretty simple to decide Human linkage mapping A 2 A 1 B 2 B 1 A 2 A 1 B 2 B 1 A 1 A 1 B 1 B 1 A 1 A 1 B 2 B 1 A 2 A 1 B 2 B 1 A 1 A 1 B 1 B 1 A 2 A 1 B 1 B 1 A 2 A 1 B 2 B 1
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So in this pedigree it is pretty simple to decide Human linkage mapping A 2 A 1 B 2 B 1 A 2 A 1 B 2 B 1 A 1 A 1 B 1 B 1 A 1 A 1 B 2 B 1 A 2 A 1 B 2 B 1 A 1 A 1 B 1 B 1 A 2 A 1 B 1 B 1 A 2 A 1 B 2 B 1 The A 2 B 2 must have come from the father’s chromosome and A 1 B 1 came from mother and are cis
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So in this pedigree it is pretty simple to decide that there are two recombinant children Human linkage mapping A 2 A 1 B 2 B 1 A 2 A 1 B 2 B 1 A 1 A 1 B 1 B 1 A 1 A 1 B 2 B 1 A 2 A 1 B 2 B 1 A 1 A 1 B 1 B 1 A 2 A 1 B 1 B 1 A 2 A 1 B 2 B 1 The A 2 B 2 must have come from the father’s chromosome and A 1 B 1 came from mother and are cis
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So in this pedigree it is pretty simple to decide that there are two recombinant children Human linkage mapping A 2 A 1 B 2 B 1 The A 2 B 2 must have come from the father’s chromosome and A 1 B 1 came from mother and are cis
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6 Please answer this question without talking to your neighbor Please don’t whisper or share answers for this question, we promise you will get a chance to talk to your neighbor!!
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Human linkage mapping One person for this dominant trait in generation III is recombinant - who?
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