Lecture%204

Lecture%204 - Cutting Heavy Chain Fab 2 Fab Fragments Are...

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Lecture 4
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Restrictions on Lateral Mobility of Plasma Membrane Proteins
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Human Red Blood Cells
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Disease May Alter RBC Morphology
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Red Blood Cells in a Capillary Normal cells Sickle cells
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Disease- Related RBC Morphologies
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Effects of Altered RBC shape RBC shape can be altered by disease or metabolic change. This in turn can lead to: Reduced ability to carry oxygen Decreased movement through capillaries Increased chance of breakage
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Comparison of Erythrocytes and Ghosts RBC Ghosts
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Scanning Electron Micrographs of RBC Ghosts and Shells Non-flattened ghost Flattened ghost Shells
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SDS Polyacrylamide Gel of Proteins in Human RBC Membrane (Ghost)
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Spectrin Molecules of Red Blood Cell Membrane
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Lateral Distribution of Band 3 is affected by Spectrin
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Freeze-fracture reveals that trypsin, a protease, alters the distribution of integral RBC proteins
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A ‘Typical’ Antibody Molecule
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Fab2 fragments are Generated by
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Unformatted text preview: Cutting Heavy Chain Fab 2 Fab Fragments Are Generated by Cutting Light Chains Fab Fab Spectrin Binding to Band 3 Binding is abolished by high salt or low pH Spectrin binds to cytoplasmic surface of RBC, which is abolished by trypsin Binding requires ankyrin, which binds to spectrin, joining it to Band 3 dimer Actin and Band 4.1 also bind spectrin COOH COOH NH 2 NH 2 Ankyrin actin actin 4.1 4.1-- Ankrin binds to junction of spectrin dimers-- Actin + band 4.1 (as well as adducin and tropomyosin) bind to ends of spectrin ab dimers Spectrin-Based Cytoskeleton on Cytosolic Side of RBC Plasma Membrane Cytoskeletal-membrane protein interactions on the cytosolic surface of erythrocytes Electron micrograph Model Subunit Structure of Integrin Cell Surface Matrix Receptor Co-alignment of Extracellular Fibronectin and Intracellular Actin...
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Lecture%204 - Cutting Heavy Chain Fab 2 Fab Fragments Are...

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