Cl07ppt - MCDB 4650 Unit 2: Induction and fate...

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MCDB 4650 lass : Axis etermination in Unit 2: Induction and fate determination Class 7: Axis determination in Xenopus Learning Goals: Explain how the D-V axis is established in Xenopus, including e timing of this event and the molecules involved the timing of this event and the molecules involved. Explain how the organizer was shown to be capable of inducing the dorsal axis. ompare he role and origin of the iewkoop enter to the role Compare the role and origin of the Niewkoop center to the role and origin of the Organizer
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The axes of the Xenopus embryo from dorsal animal blastula to gastrula stages P A dorsal ventral dorsal ventral otice that the A- axis forms as a result of the movements of astrulation vegetal Notice that the A P axis forms as a result of the movements of gastrulation
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Establishment of A-V and D-V axes in Xenopus depends upon maternally contributed components - axis: determined before A V axis: determined before fertilization by maternal mRNAs that are asymmetrically localized in the oocyte This picture shows Vg1 mRNA (encodes for a ligand) localized to the vegetal pole nother important factor is egT transcription factor) Another important factor is VegT (a transcription factor)
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hat about the D- axis? 1 st cleavage bisects the “gray crescent” What about the D V axis?
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The two sides of the embryo look different ne side has a “grey crescent” One side has a grey crescent The grey crescent side has a different potential than the other side Alter cleavage plane gpn
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V irradiation disrupts the development of the dorsal side UV irradiation disrupts the development of the dorsal side No D-V axis; No dorsal structures!
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This note was uploaded on 02/25/2010 for the course MCDB 4650 taught by Professor Staff during the Spring '08 term at Colorado.

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Cl07ppt - MCDB 4650 Unit 2: Induction and fate...

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