Cl08ppt - MCDB 4650 Class 8 The Nieuwkoop center, the...

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MCDB 4650 Class 8 The Nieuwkoop center, the Organizer, and the induction of mesoderm: molecules Learning goals: Design experiments that prove the role of mesoderm-inducing factors. Propose outcomes of experiments that prevent normal mesodermal patterning. elate the concept of orphogens o the determination of mesoderm Relate the concept of morphogens to the determination of mesoderm and the role of the organizer in Xenopus
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ingle cell Single cell Cortical rotation induced by sperm entry shifts the maternal factor Dsh to one side Vg 1 already present before cleavage Dsh protein diffuses out from cortex onto the future dorsal side of the embryo leavage cleavage Only some cells will contain Vg 1 and Dsh. Only cells with Dsh will localize β -catenin cleavage-blastula Niewkoop center is established on dorsal vegetal side in the cells that contain β -catenin and Vg1
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At early blastula, the commitment map (left) and fate aps (right) are not completely quivalent maps (right) are not completely equivalent Xenopus, early blastula stage The reason? Many signaling events still have to happen
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cientists had identified three classes of mesoderm: Scientists had identified three classes of mesoderm: Dorsal Intermediate Ventral What molecules induce mesoderm? Wm u u m m From previous experiments: elieved the signal was diffusible believed the signal was diffusible believed the signal came from vegetal cells Find candidate proteins from the vegetal cells
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What should the assays be?
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Candidate molecule: activin (a TGF β family ligand) V D Activin? TGF-b family of molecules (BMP, Nodal, TGF-b, activin) all bind to TGF-b receptor families (dimer of pe1 and type 2 subunits) type1 and type 2 subunits)
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Candidate molecule: activin (a TGF β family ligand) In the experiment below, activin containing beads were placed in the middle of animal cap cells isolated from a blastula stage embryo.
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Cl08ppt - MCDB 4650 Class 8 The Nieuwkoop center, the...

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