Cl09ppt - CDB 4650 Class 9 MCDB 4650 Class 9 The roles of...

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Unformatted text preview: CDB 4650 Class 9 MCDB 4650 Class 9 The roles of the organizer Induction of neural fates Distinguish between neural induction, segregation and commitment to different fates Explain how the organizer influences formation of neural ectoderm and termediate mesoderm including the molecules involved in these events intermediate mesoderm, including the molecules involved in these events. Compare the signaling pathways known to be involved in further induction of neural cell fate along the A-P axes with other roles that these molecules have in development. Describe the importance of lateral inhibition in neural fate determination Deduce phenotypes of embryos with deficits in these signaling molecules ow does the organizer induce the dorsal axis How does the organizer induce the dorsal axis (basically the neural ectoderm)? Original experiments showed that: the animal cap made pidermis (not neural So, scientists epidermis (not neural cells) in culture looked for a neural inducer an 3 candidates: Noggin, Chordin, Follistatin expressed in dorsal blastopore lip and notochord ncreased concentrations Increased concentrations of noggin increased the amount of dorsal structures owever when e GFb eceptor is blocked in However, when the TGFb receptor is blocked in oocytes, and then the animal cap explanted, the cells did something unexpected Mutant TGFb receptor mRNA (dominant negative) eural tissue Neural tissue Another experiment supported these findings: when animal cap cells were dissociated in culture, they made neural tissue on their own hat could be responsible for these results? Why What could be responsible for these results? Why does dissociated animal cap behave differently than explanted animal cap? The default fate of the ectoderm is neural he organizer The organizer induces by inhibiting an hibitor Sanes, Reh and Harris, 2000 inhibitor (BMPs) ummary of Organizers effects...
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This note was uploaded on 02/25/2010 for the course MCDB 4650 taught by Professor Staff during the Spring '08 term at Colorado.

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Cl09ppt - CDB 4650 Class 9 MCDB 4650 Class 9 The roles of...

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