problemset2 - MCDB 4650 Problem Set 2 1.5 Where are the...

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MCDB 4650 Problem Set 2 1. (.5) Where are the protein components of the early cleavage-stage embryo in C. elegans made (or Xenopus, the answer is the same)? i. All are synthesized in the oocyte during oogenesis. ii. Some are synthesized in the oocyte, and some in the early embryo after fertilization. iii. All are synthesized in the early embryo after fertilization. 2. (.5) Where are the transcripts made that encode these proteins? i. All are made in the oocyte during oogenesis. ii. Some are made in the oocyte, and some in the early embryo after fertilization. iii. All are made in the early embryo after fertilization. 3. (1) In C. elegans , when you separate the AB and P1 cells at the two-cell stage, neither can form a complete larva. In humans, identical twins can form up to the 16 cell stage by splitting of the embryo into two separate cell clusters. Moreover, individual human embryonic cells (blastomeres) up until about the same stage can be separated and cultured individually in a dish and still make a complete embryo. What do these results suggest about differences in the characteristics of early blastomeres between humans and C. elegans ?
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