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Unformatted text preview: 7. Suppose you used site-directed mutagenesis to create a mutation in a gene that you are interested in studying. Describe the steps you would take to clone this mutant gene into a plasmid. 8. After you clone your mutant gene into the necessary vector, you decide to perform a transformation into yeast (in an attempt to introduce this mutation into the yeasts genomic DNA). In order to see if this worked, you isolate the yeast genomic DNA and amplify your gene of interest. How would you amplify the gene and how does this process work? 9. After you amplify the gene, you want to sequence the amplified DNA to see if your mutation is present. Describe the sequencing process. 10. What is a library? What is the difference between a genomic library and a cDNA library? 11. What is a yeast two-hybrid analysis? 12. What are the three cloning vectors used in ecoli, and describe them...
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