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Lecture 21, 22 REVIEW

Lecture 21, 22 REVIEW - 7 Suppose you used site-directed...

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Lecture 21-22 Review Sheet Joshua Lai 1.For each of the following conditions, what is the transcription level of the lac operon and why? a. Low [glucose]; low [lactose] b. Low [glucose]; high [lactose] c. High [glucose]; high [lactose] 2. A repressor regulates both the lac operon and the trp operon, but not in the same way. How are the actions of the repressors different? 3. A process termed attenuation also regulates the trp operon. How does this process regulate transcription? 4. Eukaryotic transcription is generally different from prokaryotic transcription in that translational levels are typically low and activation is required to stimulate gene expression. In that light, what are the three classes of proteins involved in the activation of eukaryotic transcription? Discuss the role of each class. 5. What role, if any, does repression play in the regulation of eukaryotic transcription? 6. How are the genes involved in galactose metabolism coordinately regulated?
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Unformatted text preview: 7. Suppose you used site-directed mutagenesis to create a mutation in a gene that you are interested in studying. Describe the steps you would take to clone this mutant gene into a plasmid. 8. After you clone your mutant gene into the necessary vector, you decide to perform a transformation into yeast (in an attempt to introduce this mutation into the yeasts genomic DNA). In order to see if this worked, you isolate the yeast genomic DNA and amplify your gene of interest. How would you amplify the gene and how does this process work? 9. After you amplify the gene, you want to sequence the amplified DNA to see if your mutation is present. Describe the sequencing process. 10. What is a library? What is the difference between a genomic library and a cDNA library? 11. What is a yeast two-hybrid analysis? 12. What are the three cloning vectors used in ecoli, and describe them...
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Lecture 21, 22 REVIEW - 7 Suppose you used site-directed...

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