2 - Mass Spectrometry - SCH3U7/4U7 Mass Spectrometry A mass...

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SCH3U7/4U7 Mass Spectrometry A mass spectrometer is an instrument which separates particles according to their masses and records the relative proportions of these. The following is a simple diagram of a mass spectrometer. Region A contains the vapourized substance. Therefore, if the substance is a solid or a liquid, it has to be heated to produce the vapour. The transfer of the substance into the body of the instrument occurs very slowly to ensure there are no collisions with other particles. In Region B , the neutral atoms or molecules of the substance are bombarded by fast moving electrons which have been accelerated in an electric field. These electrons collide with electrons in the neutral substance, knocking them out, and leaving a positive ion. Region C is where the positive ions are accelerated by a high electrical potential difference. These fast moving ions enter a magnetic field at Region D produced by an electromagnet. This causes the fast moving ions to deflect. The amount of deflection experienced by an ion is dependent on its size. The larger the mass of the ion, the less the deflection (and vice versa for smaller ions). Ultimately, the deflection depends on the charge-to-mass ratio (m/z).
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This note was uploaded on 02/25/2010 for the course SCI 4u0 taught by Professor Science during the Spring '10 term at Abraham Baldwin Agricultural College.

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2 - Mass Spectrometry - SCH3U7/4U7 Mass Spectrometry A mass...

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