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Unformatted text preview: bind beGer to transi1on states than to substrates bad enzyme that just gets stuck in energy well because is more stable than products Binding energy contributes to catalysis in mul1ple ways: constrained into a certain orientation for reaction to occur • Entropy reducEon • Substrate desolvaEon • Induced ﬁt gets rid of shell of solvent around the substrate for reaction to occur Fig. 8-17 1 Substrates enter active site; enzyme changes shape such that its active site 2 Substrates held in active site by weak enfolds the substrates (induced fit). interactions, such as hydrogen bonds and ionic bonds. Enzyme-substrate complex Substrates 3 Active site can lower EA and speed up a reaction. 6 Active site is available for two new substrate molecules. Enzyme Products are 5 released. Products 4 Substrates are converted to products. Speciﬁc cataly1c groups contribute to catalysis • General acid‐base catalysis shuttling proteins
around • Covalent catalysis • Metal ion catalysis side chains coordinate with metals Eﬀects of Local CondiEons on Enzyme AcEvity • An enzyme’s acEvity can be aﬀected by – General environmental factor...
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