20 TestBank - It is theoretically possible for a gene from...

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It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible? Yes since all of them use the same genetic code. What are the essential characteristics of a cloning vector? 1. They have small size for making DNA isolation and manipulation easier 2. Independent Origin of Replication 3. They have multiple copy allowing for amplification of cloned DNA. 4. Selectable markers for the identification of the cells containing the cloning vector or recombinant clone. Plasmids are important in biotechnology because they are a way to transfer genes around. Which enzyme was used to produce the molecule in the figure at right? Restriction Enzyme. The segment of DNA shown in the figure above has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below would represent the separation and identity of these fragments? A) B) C) D) E) Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to e) cut the DNA again with restriction  endonuclease Y and insert these fragments into the plasmid cut with the same enzyme. Surprisingly, the human genome contains fewer genes than expected. How, then, is the human genome more complex than that of other organisms? A)RNA transcripts of human genes are more likely to experience alternative splicing. B)Post-translational processing adds diversity to the resulting polypeptides. C)Polypeptide domains are combined in a variety of ways. D)Different combinations of exons are used. E)All of the above are correct. What are the steps of colony hybridization? What is the genetic function of restriction enzyme? Genetic engineering is being used by the pharmaceutical industry. Which of the following is not currently one of the uses? creation of products that wil remove poisons from the human body What is a cloning vector? an agent, such as a plasmid, used to transfer DNA from an in vitro (test-tube) solution  into a living cell From the list below, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
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III, II, IV, V, I How can PCR aid in disease diagnosis? It can be used to amplify DNA from single embryonic cells for rapid prenatal diagnosis of genetic disorders; and DNA of viral genes
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This note was uploaded on 02/28/2010 for the course BIOMIC Bio765 taught by Professor Sangte during the Spring '10 term at Eastern Oregon.

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20 TestBank - It is theoretically possible for a gene from...

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