20TB - Biology 115 Fall 2004 Chapter 20 Test Bank It is...

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Biology 115 Fall 2004 1 Chapter 20 Test Bank It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible? What are the essential characteristics of a cloning vector? Plasmids are important in biotechnology because they are Which enzyme was used to produce the molecule in the figure at right? The segment of DNA shown in the figure above has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below would represent the separation and identity of these fragments? A) B) C) D) E) Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to
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Biology 115 Fall 2004 2 Surprisingly, the human genome contains fewer genes than expected. How, then, is the human genome more complex than that of other organisms? What are the steps of colony hybridization? What is the genetic function of restriction enzyme? Genetic engineering is being used by the pharmaceutical industry. Which of the following is not currently one of the uses? What is a cloning vector? From the list below, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III.Extract plasmid DNA from bacterial cells IV.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA How can PCR aid in disease diagnosis? Bacteria containing recombinant plasmids are often identified by which process? Use the following information to answer the four questions below. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes, which make it resistant to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are
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20TB - Biology 115 Fall 2004 Chapter 20 Test Bank It is...

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