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ch 20 quiz - 1 When a typical restriction enzyme cuts a DNA...

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1. When a typical restriction enzyme cuts a DNA molecule, the cuts are staggered so that the DNA fragments have single-stranded ends. This is important in recombinant DNA work because _____. (p. 377) ___it allows a cell to recognize fragments produced by the enzyme ___the single-stranded ends serve as starting points for DNA replication ___the fragments will bond to other fragments with complementary single-stranded ends ___it enables researchers to use the fragments as introns ___only single-stranded DNA segments can code for proteins 2. Scientists wished to create an organism capable of breaking down several kinds of toxic wastes, so they combined genes from several species of bacteria to create a single superbacterium. They probably did NOT need to use which of the following? (p. 376) ___nucleic acid probes ___F factors ___plasmids ___restriction enzymes ___DNA ligase 3. DNA used in recombinant DNA techniques is first cut into fragments by _____. (p. 377) ___enzymes called ligases ___restriction enzymes ___treatment with DNA polymerase ___treatment with a strong acid or base ___X-rays 4. "Sticky ends" are very useful in genetic engineering because _____. (p. 377) they provide a site for complementary base pairing so that pieces of DNA can be linked together they prevent the enzymatic degradation of engineered DNA they allow scientists to label genes in a living cell they allow scientists to use the edited form of mRNA for genetic engineering they serve as the means for getting recombinant DNA into another cell, such as a bacterium 5. An enzyme that "cuts" DNA at a symmetrical sequence of bases is called _____. (p. 377) reverse transcriptase restriction enzyme cDNA palindrome ligase
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6. Bacteria use restriction enzymes to _____. (p. 377) synthesize DNA synthesize RNA synthesize protein destroy foreign protein destroy foreign DNA 7. Which one of the following enzymes could seal a nick in one strand of a double-stranded DNA molecule by creating a sugar-phosphate bond between the adjacent, unjoined nucleotides? (p. 377) DNA ligase everse transcriptase restriction enzyme terminator enzyme DNA polymerase 8. What two enzymes are needed to produce recombinant DNA? (p. 377) a restriction enzyme and a topoisomerase a restriction enzyme and a ligase a restriction enzyme and a polymerase a polymerase and a ligase a polymerase and a topoisomerase 9. Restriction enzymes leave "sticky ends" when they cut a piece of DNA. The most useful result of this is that the sticky ends allow ____________. (p. 377) the cut to be easily repaired by ligases the easy insertion of a foreign piece of DNA that has had its ends cut by the same restriction enzyme the cutting of a piece of DNA without the separation of the two strands the creation of a hybrid molecule in which one strand is DNA and the other strand is RNA all of the above 10. In genetic engineering "sticky ends" refers to _________. (p. 377) a technique for finding a gene of interest within a nucleus without destroying the cell the ability of plasmids to stick to a bacterial cell wall and thus be taken up into the bacterium
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