ch 20 - Chapter 20 MULTIPLE CHOICE Choose the one...

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Chapter 20 MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible? A)All organisms have the same genetic code. B)All organisms are made up of cells. C)All organisms have similar nuclei. D)All organisms have ribosomes. E)All organisms have transfer RNA. 2) One important approach to gene cloning uses A)whole chromosomes. B)plasmids. C)noncoding nucleotide sequences. D)bacteria. E)B and D above. 3) Current applications of gene cloning include A)cleaning up toxic waste. B)instilling pest resistance in plants. C)manufacturing human growth hormone. D)A and C only. E)A, B, and C. 4) Plasmids are important in biotechnology because they are A)a vehicle for the insertion of recombinant DNA into bacteria. B)recognition sites on recombinant DNA strands. C)surfaces for protein synthesis in eukaryotic recombinants. D)surfaces for respiratory processes in bacteria. E)proviruses incorporated into the host DNA. 5) If you discovered a bacterial cell that contained no restriction enzymes, which of the following would you expect to happen? A)The cell would be unable to replicate its DNA. B)The cell would create incomplete plasmids. C)The cell would be easily infected and lysed by bacteriophages. D)The cell would become an obligate parasite. E)Both A and D would occur. Figure 20.1 6) Which enzyme was used to produce the molecule in Figure 20.1? A)ligase B)transcriptase C)a restriction enzyme D)RNA polymerase E)DNA polymerase 7) Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to A)insert the fragments cut with X directly into the plasmid without cutting the plasmid. B)cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid. C)cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme. D)cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the human DNA fragments cut with restriction enzyme X. E)cut the plasmid with enzyme X and then insert the gene into the plasmid.
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8) What is the genetic function of restriction enzyme? A)adds new nucleotides to the growing strand of DNA B)joins nucleotides during replication C)joins nucleotides during transcription D)cleaves nucleic acids at specific sites E)repairs breaks in sugar-phosphate backbones 9) The restriction enzyme used in constructing hybrid molecules of certain gene sequences and plasmid DNA acts by A)opening DNA molecules at specific sites, leaving sticky ends exposed. B)sealing plasmid DNA and foreign DNA into a closed circle. C)transcribing plasmid DNA into a transformed
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ch 20 - Chapter 20 MULTIPLE CHOICE Choose the one...

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