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CHAP 20 TEST - AP Biology Test 13(Chapter 20 Regan 1...

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AP Biology Test 13 (Chapter 20) Name: Regan Date: 1) Plasmids are important in biotechnology because they are A) a vehicle for the insertion of foreign genes into bacteria. B) recognition sites on recombinant DNA strands. C) surfaces for protein synthesis in eukaryotic recombinants. D) surfaces for respiratory processes in bacteria. E) proviruses incorporated into the host DNA. 2) What is the enzymatic function of restriction enzymes? A) to add new nucleotides to the growing strand of DNA B) to join nucleotides during replication C) to join nucleotides during transcription D) to cleave nucleic acids at specific sites E) to repair breaks in sugar-phosphate backbones 3) What is a cloning vector? 4) What are the typical characteristics of a cloning vector? 5) What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA.
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6) Yeast cells are frequently used as hosts for cloning because A) they are easy to grow. B) they can remove introns from mRNA. C) they have plasmids. D) both A and B E) A, B, and C
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