CHAP 20 TEST - AP Biology Test 13 (Chapter 20) Regan 1)...

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AP Biology Test 13 (Chapter 20) Name: Regan Date: 1) Plasmids are important in biotechnology because they are A) a vehicle for the insertion of foreign genes into bacteria. B) recognition sites on recombinant DNA strands. C) surfaces for protein synthesis in eukaryotic recombinants. D) surfaces for respiratory processes in bacteria. E) proviruses incorporated into the host DNA. 2) What is the enzymatic function of restriction enzymes? A) to add new nucleotides to the growing strand of DNA B) to join nucleotides during replication C) to join nucleotides during transcription D) to cleave nucleic acids at specific sites E) to repair breaks in sugar-phosphate backbones 3) What is a cloning vector? A) an enzyme that cuts DNA into restriction fragments B) a DNA probe used to locate a particular gene in the genome C) an agent, such as a plasmid, used to transfer DNA from an in vitro solution into a living cell D) the laboratory apparatus used to clone genes E) the sticky end of a DNA fragment 4) What are the typical characteristics of a cloning vector? A) Bacterial cells cannot survive without it when grown under certain conditions. B) It contains restriction sites that allow the insertion of foreign DNA segments. C) It can replicate in bacterial cells. D) Only B and C are correct. E) A, B, and C are correct. 5) What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA. A) I, II, IV, III, V B) II, III, V, IV, I C) III, II, IV, V, I D) III, IV, V, I, II E) IV, V, I, II, III
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6) Yeast cells are frequently used as hosts for cloning because A) they are easy to grow. B) they can remove introns from mRNA. C) they have plasmids. D) both A and B E) A, B, and C 7) How does a genomic library differ from a cDNA library? A) A genomic library contains both noncoding sequences and coding sequences, whereas a cDNA library contains only coding sequences. B) A genomic library is identical regardless of the cell type used to make it, whereas the content of a cDNA library depends on the cell type used in its construction. C) A genomic library can be made using a restriction enzyme and DNA ligase only, whereas a cDNA library requires both of these as
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CHAP 20 TEST - AP Biology Test 13 (Chapter 20) Regan 1)...

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