AP Biology Test 13 (Chapter 20)
Plasmids are important in biotechnology because they are
A) a vehicle for the insertion of foreign genes into bacteria.
B) recognition sites on recombinant DNA strands.
C) surfaces for protein synthesis in eukaryotic recombinants.
D) surfaces for respiratory processes in bacteria.
E) proviruses incorporated into the host DNA.
What is the enzymatic function of restriction enzymes?
A) to add new nucleotides to the growing strand of DNA
B) to join nucleotides during replication
C) to join nucleotides during transcription
D) to cleave nucleic acids at specific sites
E) to repair breaks in sugar-phosphate backbones
What is a cloning vector?
A) an enzyme that cuts DNA into restriction fragments
B) a DNA probe used to locate a particular gene in the genome
C) an agent, such as a plasmid, used to transfer DNA from an
solution into a living cell
D) the laboratory apparatus used to clone genes
E) the sticky end of a DNA fragment
What are the typical characteristics of a cloning vector?
A) Bacterial cells cannot survive without it when grown under certain conditions.
B) It contains restriction sites that allow the insertion of foreign DNA segments.
C) It can replicate in bacterial cells.
D) Only B and C are correct.
E) A, B, and C are correct.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a
Transform bacteria with recombinant DNA molecule.
Cut the plasmid DNA using restriction enzymes.
Extract plasmid DNA from bacterial cells.
Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
Use ligase to seal plasmid DNA to nonplasmid DNA.
A) I, II, IV, III, V
B) II, III, V, IV, I
C) III, II, IV, V, I
D) III, IV, V, I, II
E) IV, V, I, II, III