midterm_2005

midterm_2005 - page1 (of10) BIBC102 Midterm 1 Fa 2005...

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page1 (of10) Name____________________________________ BIBC102 Midterm 1 Fa 2005 Stu. ID#__________________________________ Proteins and peptides (9 points) Behold, a license plate! It is from a custom classic car (ca. 1940) on display at Simpson’s Nursery in Jamul, which has many plants and a remarkable collection of vehicles (go figure…). 1; 4 pts) Write the sequence of the peptide spelled out in the plate, using the three letter code (eg. a tripeptide of tryptophan would be TrpTrpTrp). 2) At physiological pH (7.0), what is the net charge of this peptide? (note this only requires knowing the R groups on each amino acid) _______________ 3) Give a one sentence, general description of a prosthetic group 4) Write the name of the prosthetic group that contains iron ______________ 5; 2 pts) Write the name of the type of protein secondary structure that is depicted in the little pictures, using the space under each one _____________________ ______________________
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page2 (of10) Name____________________________________ BIBC102 Midterm 1 Fa 2005 Stu. ID#__________________________________ who’re those jerks, lineweaver and burk? they took my curve and made a line that takes some nerve so I’ll speak my mind those foolish nerds be wastin’ your time cause the hyperbola is still sublime Eminem: The Slim Substrate LP You are analyzing three enzymes (A, B and C) that catalyze the same reaction. You determine the velocity (V) at various substrate concentrations (S), and plot the data using the Lineweaver-Burk method, excoriated by Eminem (see inset box). The resulting graph is shown on the right, with each enzyme’s data indictated by letter. Answer the following questions. 6) What does the horizontal axis represent? ____________ 7) What does the vertical axis represent? ____________ 8) Which enzyme has the highest Vmax? ____________ 9) Which enzyme attains 50% Vmax at the lowest substrate concentration? ____________ 10) A fourth isozyme has a curved lineweaver burke plot. Sketch what the S vs V plot for that enzyme will look like in the space to the right. . 11) What can you say about the structure of this fourth enzyme? 12) (not Lineweaver-Burk related) Write the equation that defines kcat k cat = (Hint: this is not Km) 13) What is the definition of kcat in English? That is, use a sentence to describe its physical meaning, as opposed to an equation.
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page3 (of10) Name____________________________________ BIBC102 Midterm 1 Fa 2005 Stu. ID#__________________________________ 3 2 1 Three residues for the Enzyme-Kings… (8 points) The catalytic triad is a very common feature of many enzymes that hydrolyze chemical bonds. We focused on chymotrypsin in this class. Below are the three amino acids of the chymotrypsin catalytic triad. Name each amino acid, and in one sentence describe its function in the catalytic triad.
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midterm_2005 - page1 (of10) BIBC102 Midterm 1 Fa 2005...

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