Mcb250F09PractEx3 - MCB250 Practice Exam for Exam 3...

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MCB250 Practice Exam for Exam 3 November 4, 2009 Choose the one BEST answer. 1. During conjugation between bacteria: A. Double stranded DNA is transferred to the recipient B. Transposons present on the incoming DNA may be activated to transfer to the recipient chromosome. C. Bacteriophage components are used to build a bridge between the donor and recipient cells. D. RecA is required for the process. E. Homologous DNA but not foreign DNA can be inherited by the recipient cells. 2. The mechanisms by which replicative and non-replicative transposons transpose differ in that: A. Replicative transposons form an RNA-DNA hybrid during the replication phase of transposition. B. Non-replicative transposons use a serine/threonine-type recombinase while replicative transposons use a DDE-type recombinase. C. Replicative transposition results in fused replicons while non-replicative transposition results in a double-strand break in the donor replicon. D. Replicative transposons are bounded by direct repeats while non- replicative transposons are bounded by indirect repeats. E. The transposase complex of replicative transposons makes a flush-end double-stranded set of cuts at the target site in the recipient replicon while the transposase complex of non-replicative transposons makes a staggered set of cuts in the target site of the recipient replicon. 3. By definition, genomes: A. Are made entirely of DNA. B. Must be double-stranded nucleic acids. C. Are composed of replicating and non-replicating nucleic acid molecules in the cell. D. Include nuclear and organellar nucleic acid complements in eukaryotes. E. Include chromosomes but exclude plasmids in prokaryotes. 4. Increases in genome size seen as one moves from bacteria to animals and plants results predominantly from: A. Increases in amounts of non-coding DNA.
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B. Increases in the size of the coding regions of genes. C. Increases in the number of protein-coding genes. D. Increases in the numbers of rRNA genes. E. Increases in the numbers of chromosomes. 5. Histones: A. Preferentially are associated with centromeres where they assist in microtubule organization during mitosis and meiosis. B. Provide a structural mechanism for compacting DNA so that long DNA molecules can fit into the nucleus of the cell. C. Are characteristic of prokaryotic but not eukaryotic genomes. D. Hide origins of replication, thus controlling initiation of DNA replication. E. Interact with Rad51 to initiate double-strand breaks during meiosis. 6. Bacterial plasmids: A. Can vector DNA to other bacteria but not to cells of higher life forms. B. Must integrate into bacterial chromosomes for their replication. C. Generally are double-stranded linear DNA molecules. D.
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This note was uploaded on 03/02/2010 for the course MCB 250 taught by Professor Staff during the Spring '08 term at University of Illinois, Urbana Champaign.

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Mcb250F09PractEx3 - MCB250 Practice Exam for Exam 3...

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