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Unformatted text preview: section of DNA may not adequately distinguish among closely related species or complex groups. Moreover, in plants, there is still much debate over which gene sequence region should be used and its reliability. In animals, the 5' segment of mitochondrial cytochrome oxidase subunit I (COI) is relatively established as a barcoding marker, but Spooner highlights many groups where COI fails to distinguish species. The COI region fails completely for plants because it evolves at a slower rate in plants and has a much more variable sequence. The search for alternative barcoding regions in plants is especially problematic. Although several gene sequences have been proposed for plants, none of them serves as a universal barcode marker. Regions that have been proposed for plants include a section of the nuclear ribosomal DNA: the internal non-transcribed spacer region (ITS); and various plastid regions to include the trnH-psbA intergenic spacer and the plastid genes rpoC1, rpoB, and matK....
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This note was uploaded on 03/02/2010 for the course MATH 139 taught by Professor Tall during the Spring '08 term at SIU Carbondale.
- Spring '08