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Answer10 - MCB 161 Study problems Answer to MBOC problems S...

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MCB 161 S. Harmer Study problems Basal Transcription Machinery Answer to MBOC problems: 6-25 Phosphorylation of the CTD is the event that permits release of RNA polymerase from the other proteins present at the start point of transcription. Phosphorylation also allows association of a new set of proteins that are involved in processing the nascent RNA transcript. These proteins include components required for capping, splicing, and polyadenylation. 6-38 A. There are three locations where the linker-scanning mutations dramatically decreased the amount of transcript. These are from about –10 to –30, –45 to –60, and –80 to –105. The promoter element at –10 to –30 seems to have the most dramatic effect, whereas the other two are somewhat less and about equal to one another. You may also have noticed the effect of a linker scanning mutation that overlapped the start site of transcription. In the absence of the usual sequence at the start site, the transcript is initiated at a variety of positions. B. The segment from –10 to –30 likely includes the TATA box, which is usually located about 25 nucleotides or so upstream of the transcription start site. Reference: McKnight SL & Kingsbury R (1982) Transcriptional control signals of a eukaryotic protein-coding gene. Science 217, 316–324. 1. For each of the following statements referring to aspects of eukaryotic gene expression circle which types of genes (type I, II, or III) to which the statement applies. Circle ALL correct answers for full credit. I II III Transcription complex includes TATA-binding protein (TBP). I II III Transcription usually requires TFIID. I II III RNA product can be translated into protein. I II III RNA product can be a component of a ribosome.
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MCB 161 S. Harmer Study problems Basal Transcription Machinery 2. The diagram below illustrates the results of a gel shift experiment. Two DNA fragments, DNA 1 and DNA 2, are suspected of including binding sites for two newly discovered general transcription factors (factor X and factor Y). The fragments were radioactively labeled and used in the gel shift assay with purified factors X and Y. The information above each lane indicates which components were added to the reaction run in that lane. Migration is from top to bottom. Results for the last lane (at right) are not given.
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