Answer13 - MCB 161 Study problems S. Harmer Regulation of...

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MCB 161 S. Harmer Study problems Regulation of Transcription III 7-64 The two models make different predictions about the activities of the mutant receptors. If a DNA-binding domain were created by hormone binding, then mutant receptors lacking the hormone-binding domain would fail to activate CAT expression. By contrast, if a preexisting DNA-binding domain were uncovered by hormone binding, then some mutant receptors would be expected to activate CAT expression constitutively (in the presence or absence of hormone) due to deletion of a region of protein that masks an otherwise functional DNA-binding domain. The experimental results match the expectations for a preexisting DNA binding domain that is masked by the hormone-binding domain. The four mutants with the longest C- terminal segments (shortest deletions) are missing enough of the hormone-binding domain to interfere with hormone binding, but they still retain enough to mask the DNA- binding domain. The next four mutants, which constitutively activate CAT expression,
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Answer13 - MCB 161 Study problems S. Harmer Regulation of...

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