Answer14 - MCB 161 Study problems 6-44 S. Harmer mRNA...

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MCB 161 S. Harmer Study problems mRNA Processing 6-44 The results of these experiments argue convincingly that base-pairing occurs between the pre-mRNA and the U1 RNA. The base-pairing between the U1 snRNA and the pre- mRNA is not extensive, but it is better when splicing occurs successfully than when it does not. These experiments illustrate a classic approach for testing the reality of proposed base-pairing schemes. If base pairing is important, then a base change in one component should interfere with the process. A compensating change in the second component (to restore base-pairing) should then reestablish the process. That is just what was observed in these experiments. A change of a G A in the pre-mRNA inhibited splicing, whereas a compensating C U change in the U1 RNA, which should restore base-pairing (GC AU), reestablished splicing. Moreover, none of the other mutants of the U1 RNA (which would not restore base-pairing) overcome the splicing defect. Reference: Zhuang Y & Weiner AM (1986) A compensatory base change in U1 snRNA
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This note was uploaded on 03/02/2010 for the course MCB 161 taught by Professor Stacey during the Winter '10 term at UC Davis.

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Answer14 - MCB 161 Study problems 6-44 S. Harmer mRNA...

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