Unformatted text preview: : see the animations on the web site for help!) C. Enzymology of HR: Chromosome alignment; Spo11 generates DSB; RecA/Rad51 performs homology search and catalyzes strand exchange III. Positional cloning - molecular identification of mutation underlying a phenotype A. Unlinked genes segregate independently (independent assortment) B. Genes located near each other on a chromosome tend to be inherited together (they are linked). Physical proximity means alleles seldom separated by recombination C. Linkage analysis is used to find the approximate location of a mutant gene; fine-scale mapping using molecular markers (like SSLPs) is then used to determine the precise location of the mutant gene D. Genes in the mutant interval are then sequenced to identify the responsible locus....
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- Winter '10
- DNA, DNA repair, web site, homologous recombination, Homologous recombination a.