Note8 - responsible locus II Transposition A DNA...

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Lecture 9 MCB 161 S. Harmer 2/02/2010 Positional Cloning, Transposition, Overview of gene expression Reading Watson text, pp 319 - 320; 334 - 351; 377 - 384; box 12-1 (p 388) Problems see the web site I. Positional cloning - molecular identification of mutation underlying a phenotype A. Unlinked genes segregate independently (independent assortment) B. Genes located near each other on a chromosome tend to be inherited together (they are linked). Physical proximity means alleles seldom separated by recombination C. Linkage analysis is used to find the approximate location of a mutant gene; fine-scale mapping using molecular markers (like SSLPs) is then used to determine the precise location of the mutant gene D. Genes in the mutant interval are then sequenced to identify the
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Unformatted text preview: responsible locus II. Transposition A. DNA transposons B. Retroviruses & retrotransposons III. Overview of gene expression IV. Bacterial gene expression A. Transcription and transcription signals 1. RNA polymerase holoenzyme = core ( α 2 , ß, ß’) + σ . 2. Polymerase recognizes genes by presence of specific promoter sequences. 3. Operator regions (specific DNA sequences) regulate expression of many genes. 4. Transcription terminates at specific sites where stem-loop structures form in RNA. B. Translation in prokaryotes 1. Requires ribosome, tRNA, aminoacyl-tRNA synthase and other factors. 2. Specific ribosome binding site proximal to AUG start codon. 3. Co-translational, polycistronic mRNA common....
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