Note9 - Lecture 10 S Harmer Overview of gene expression MCB...

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Lecture 10 MCB 161 S. Harmer 2/04/2010 Overview of gene expression Reading Watson text, pp 377 – 384; 388 (box 12-1); 396 – 412; 415 – 416; 457 – 458; 777 - 778 Problems see the web site I. Overview of gene expression II. Bacterial gene expression A. Transcription and transcription signals 1. RNA polymerase holoenzyme = core ( α 2 , ß, ß’) + σ . 2. Polymerase recognizes genes by presence of specific promoter sequences. 3. Operator regions (specific DNA sequences) regulate expression of many genes. 4. Transcription terminates at specific sites where stem-loop structures form in RNA. B. Translation in prokaryotes 1. Requires ribosome, tRNA, aminoacyl-tRNA synthase and other factors. 2. Specific ribosome binding site proximal to AUG start codon. 3. Co-translational, polycistronic mRNA common. III. Eukaryotic gene expression A. Three polymerases (but a fourth and fifth found recently in plants!) 1. RNA polymerase I (pol I) – transcribes rRNA precursor which is processed to 5.8S,
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Note9 - Lecture 10 S Harmer Overview of gene expression MCB...

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