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Unformatted text preview: (Myc, Max, OCT-2) Helix-Loop-Helix - Dimerization and DNA binding, MyoD, Myc, Max, Ig enhancer-binding, daughterless Other - MADS, Fungal Finger (Zn Cluster), unclassified III. Identification and Isolation of Transcription Factors A. Purification by standard biochemical methods, assay by footprint or gel shift (EMSA) B. Genetic identification - mutations affecting a whole process C. Several viral oncogenes and their cellular homologs have been found to be transcription factors. Lecture 12 MCB 161 S. Harmer 2/11/10 From Singh (1998) Plant Physiology, 118:1111-1120 From Gutman & Wasylyk (1991) Trends in Genetics, 7: 49-54 Figure 12-18 Regulatory transcription factors (the blue activators in the figure) may bind to a co-activator such as the Mediator complex or to chromatin remodeling factors (like Swi/Snf and/or histone acetyl transferases)....
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