Note11 - (Myc, Max, OCT-2) Helix-Loop-Helix - Dimerization...

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Lecture 12 MCB 161 S. Harmer 2/11/10 Regulation of Transcription I Reading: Watson: p. pp 589 – 603 Problems: See web site. I. Promoters and Enhancers/Silencers A. Enhancers and silencers – DNA sequences that promote or repress transcription independent of orientation; can be upstream or downstream of promoter B. Binding sites for regulatory transcription factors. C. Most genes have multiple enhancers and silencers - allows for complex regulation. II. Properties of Regulatory Transcription Factors A. General properties Domain structure - binding, dimerization, activation Specific binding sites, some palindromic B. Activation domains Acidic, amphipathic helix G ln-rich Pro-rich C. Binding/dim erization domains Helix-Turn-Helix - homeodomains Zn-finger - Cys/His; Cys - steroid hormone receptors Rational design of C2H2 transcription factors. Leucine Zipper (bZip, bCC) - Dimerization, Jun, Fos, GCN4, C/EBP, CREB,
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Unformatted text preview: (Myc, Max, OCT-2) Helix-Loop-Helix - Dimerization and DNA binding, MyoD, Myc, Max, Ig enhancer-binding, daughterless Other - MADS, Fungal Finger (Zn Cluster), unclassified III. Identification and Isolation of Transcription Factors A. Purification by standard biochemical methods, assay by footprint or gel shift (EMSA) B. Genetic identification - mutations affecting a whole process C. Several viral oncogenes and their cellular homologs have been found to be transcription factors. Lecture 12 MCB 161 S. Harmer 2/11/10 From Singh (1998) Plant Physiology, 118:1111-1120 From Gutman & Wasylyk (1991) Trends in Genetics, 7: 49-54 Figure 12-18 Regulatory transcription factors (the blue activators in the figure) may bind to a co-activator such as the Mediator complex or to chromatin remodeling factors (like Swi/Snf and/or histone acetyl transferases)....
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This note was uploaded on 03/02/2010 for the course MCB 161 taught by Professor Stacey during the Winter '10 term at UC Davis.

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Note11 - (Myc, Max, OCT-2) Helix-Loop-Helix - Dimerization...

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