Problem10 - MCB 161 Study problems S Harmer Basal...

MCB 161 S. Harmer
Study problems Basal Transcription Machinery
Problems 6-25 and 6-38 from the MBOC problems book; plus, answer the below
questions.
1. For each of the following statements referring to aspects of eukaryotic gene expression
circle which types of genes (type I, II, or III) to which the statement applies. Circle ALL
correct answers for full credit.
I II III Transcription complex includes TATA-binding protein (TBP).
I II III Transcription usually requires TFIID.
I II III RNA product can be translated into protein.
I II III RNA product can be a component of a ribosome.
2. The diagram below illustrates the results of a gel shift experiment. Two DNA
fragments, DNA 1 and DNA 2, are suspected of including binding sites for two newly
discovered general transcription factors (factor X and factor Y). The fragments were
radioactively labeled and used in the gel shift assay with purified factors X and Y. The
information above each lane indicates which components were added to the reaction run
in that lane. Migration is from top to bottom. Results for the last lane (at right) are not
given.
A. For each of the following circle “T” if the statement is true, “F” if the statement is