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Problem11 - MCB 161 Study problems S Harmer Regulation of...

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MCB 161 S. Harmer Study problems Regulation of Transcription I & II The assignments for these two lectures are the below problems, and in addition MBOC problems 7-29, 7-35, 7-52, and 7-63. Also, be sure you understand Fig. 3, 5, 6, and 7 in Blackwood paper (available on web site). 1. Researchers studying transcription of a human gene constructed a set of plasmids containing various mutations in the promoter region of this gene. The plasmids were assayed to determine the level of transcription in both an in vitro system (containing partially fractionated human cell nuclear extracts), and in transgenic cells. The following data were obtained, with a level of “1” representing transcription from the unmutated promoter in each system (even though the level of transcription was at least 10-fold higher in vivo than in vitro): a) (6) What types of sequence elements are likely to be in the promoter near positions –25 and –75 where mutations seem to have strong affects in both systems?
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  • Winter '10
  • STACEY
  • DNA, Transcription, Essential amino acid, Transcription factor, Ile Ala Ala Ser Lys Cys Arg Lys Arg Lys Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Gln Glu Gln Thr Pro Gln Arg Gln Gln Asn Gln Thr Ser Gly Gly Ser Leu, Arg Asn Arg

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