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Problem14 - student expect the sequence changes to...

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MCB 161 S. Harmer Study problems mRNA Processing The below problems plus MBOC 6-44 and 7-102 1. A student has identified a new plant gene by insertional mutagenesis (getting a mutant by randomly inserting transposons or transforming DNA in the genome). The student was then able to isolate the gene with the insert inserted into the coding region (shown below). The student wants to express the gene in transgenic plants. In an attempt to directly use the currently cloned interrupted gene the student engineers a "GT" at the 5'- end (relative to transcription) of the insert and an "AG" at the 3'-end of the insert. a) (2) Why were these sequences engineered at the ends of the insert, and what did the
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Unformatted text preview: student expect the sequence changes to accomplish? b) (4) Do you think this approach is likely to work? Why or why not? 2. (2) Some pre-mRNAs (a class of "group II" introns, mostly in organelles) are self-splicing. That is, these pre-mRNAs contain sequences in their introns which catalyze the cleavages necessary for intron removal. In what location would you expect to find RNA sequences which would possibly perform similar roles in aiding catalysis of the corresponding reactions for pre-mRNAs which are not self-splicing, i.e. as is the case for most nuclear encoded mRNAs (be specific in your answer)?...
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