fplc_sp10 - and elution with 1 M NaCl 2) Carry out the...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
CH 369T Spring 2010 Tutorial: FPLC Purification of Proteins Rationale: Learn basic operation of fast protein liquid chromatography (FPLC) instrumentation, choice of columns, etc. FPLC uses the same or similar column chemistries as you have used with low-pressure column chromatography of proteins, but the automation makes the system more flexible and easier to use. Objectives: 1) Create a method for separation of a mixture of ovalbumin and lysozyme, in pH 7.0 phosphate buffer, using FPLC with either cation or anion exchange columns, as assigned,
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: and elution with 1 M NaCl 2) Carry out the separation using this method and protein mixture supplied 3) Write a brief report including the type of column used, buffer compositions and gradient profile, and a chromatogram with peaks identified FPLC ion exchange column assignments: Group A/B/C: Carboxymethyl (CM) Group D/E/F: Diethylaminoethyl (DEAE) FPLC Schedule: Group Date A, C, E Jan. 26 B, D, F Jan. 28 1...
View Full Document

This note was uploaded on 03/03/2010 for the course CH 371K taught by Professor Mcdonald during the Spring '10 term at University of Texas at Austin.

Ask a homework question - tutors are online