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fplc_sp10 - and elution with 1 M NaCl 2 Carry out the...

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CH 369T Spring 2010 Tutorial: FPLC Purification of Proteins Rationale: Learn basic operation of fast protein liquid chromatography (FPLC) instrumentation, choice of columns, etc. FPLC uses the same or similar column chemistries as you have used with low-pressure column chromatography of proteins, but the automation makes the system more flexible and easier to use. Objectives: 1) Create a method for separation of a mixture of ovalbumin and lysozyme, in pH 7.0 phosphate buffer, using FPLC with either cation or anion exchange columns, as assigned,
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Unformatted text preview: and elution with 1 M NaCl 2) Carry out the separation using this method and protein mixture supplied 3) Write a brief report including the type of column used, buffer compositions and gradient profile, and a chromatogram with peaks identified FPLC ion exchange column assignments: Group A/B/C: Carboxymethyl (CM) Group D/E/F: Diethylaminoethyl (DEAE) FPLC Schedule: Group Date A, C, E Jan. 26 B, D, F Jan. 28 1...
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