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rt_purif_sp10_obj - You will deliver(1 at least 100 units...

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CH 369T Spring 2010 Expression and Purification of Cloned Reverse Transcriptase Reference: RT purification protocol, RT assay protocol Rationale: Reverse transcriptase is used in molecular biology to generate cDNA copies of mRNA for cloning purposes. This gene has been inserted into an expression vector, under control of an IPTG-inducible promoter, and transformed into E. coli . You will express, isolate and purify the recombinant reverse transcriptase. Objective:
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Unformatted text preview: You will deliver (1) at least 100 units of reverse transcriptase with an estimated specific activity of not less than 20 units per mg, purified by the protocols referenced above, (2) a report including data from induction and purification procedures, enzyme assays, and polyacrylamide gel electrophoresis. One enzyme unit is defined here as the amount of enzyme that will catalyze the incorporation of 1 nmol dTTP in 10 min. at 37°C....
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