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Unformatted text preview: You will deliver (1) at least 100 units of reverse transcriptase with an estimated specific activity of not less than 20 units per mg, purified by the protocols referenced above, (2) a report including data from induction and purification procedures, enzyme assays, and polyacrylamide gel electrophoresis. One enzyme unit is defined here as the amount of enzyme that will catalyze the incorporation of 1 nmol dTTP in 10 min. at 37C....
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This note was uploaded on 03/03/2010 for the course CH 371K taught by Professor Mcdonald during the Spring '10 term at University of Texas at Austin.
- Spring '10