Lec8.2010 - BIO 320 CELL BIOLOGY T O'Halloran A De Lozanne...

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BIO 320 - CELL BIOLOGY Lecture 11 (02/11/20010). Golgi Transport, the Trans-Golgi Network Reading: Alberts, 5th Ed. Pages 779-798 I. Vesicular transport within the Golgi complex - how do materials move through the Golgi ? Experimental evidence for vesicle traffic A. Different experimental systems used to study membrane traffic 1. yeast secretory mutants pioneered by Randy Shekman’s lab and others 2. Biochemical reconstitution pioneered by Jim Rothman B. Maturation model - cisternae are transient structures; form at cis face by ER vesicle fusion, travel to trans face & altered along the way; cisternae mature as move through 1. Evidence - algal covered by scales thus produce unusually large secretory products; seen within Golgi cisternae but not in small transport vesicles C. Vesicular transport - cisternae remain in place as stable entities held together by protein scaffold; 1. Evidence - cell-free reconstitution of vesicle transport in systems from Chinese hamster ovary (CHO) a. Donor fraction infected with vesicular stomatitis virus (VSV) that encodes integral protein (VSV-G) b. Donor also mutant for N-acetylglucosamine transferase (sugar-transferring enzyme) found in medial cisternae c. Acceptor fraction from uninfected, wild-type cells has N-acetylglucosamine transferase
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This note was uploaded on 03/03/2010 for the course BIO 320 taught by Professor Staff during the Spring '08 term at University of Texas.

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Lec8.2010 - BIO 320 CELL BIOLOGY T O'Halloran A De Lozanne...

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