Lec8.2010 - BIO 320 - CELL BIOLOGY T. O'Halloran & A....

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BIO 320 - CELL BIOLOGY Lecture 11 (02/11/20010). Golgi Transport, the Trans-Golgi Network Reading: Alberts, 5th Ed. Pages 779-798 I. Vesicular transport within the Golgi complex - how do materials move through the Golgi ? Experimental evidence for vesicle traffic A. Different experimental systems used to study membrane traffic 1. yeast secretory mutants pioneered by Randy Shekman’s lab and others 2. Biochemical reconstitution pioneered by Jim Rothman B. Maturation model - cisternae are transient structures; form at cis face by ER vesicle fusion, travel to trans face & altered along the way; cisternae mature as move through 1. Evidence - algal covered by scales thus produce unusually large secretory products; seen within Golgi cisternae but not in small transport vesicles C. Vesicular transport - cisternae remain in place as stable entities held together by protein scaffold; 1. Evidence - cell-free reconstitution of vesicle transport in systems from Chinese hamster ovary (CHO) a. Donor fraction infected with vesicular stomatitis virus (VSV) that encodes integral protein (VSV-G) b. Donor also mutant for N-acetylglucosamine transferase (sugar-transferring enzyme) found in medial cisternae c. Acceptor fraction from uninfected, wild-type cells has N-acetylglucosamine transferase
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

Lec8.2010 - BIO 320 - CELL BIOLOGY T. O'Halloran & A....

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online