chem bio full note

chem bio full note - Department of Department of 1...

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Unformatted text preview: Department of Department of 1 Chemistry Chemistry CHEM 1AA3: Intro. Chemistry II Chemical Biology Brainwashing Bees , Drug Discovery, and Aromaticity 2 Chem 1AA3 Queen Pheromone Blocks Aversive Learning in Young Worker Bees Vergoz et al. Science ( 2007 ) 317:384-386 queen mandibular pheromone (QMP)- causes young workers to feed and groom her- suppresses new queens, controls colony behaviour read the accompanying file, "2010_Chembio_commentary.pdf" 3 Chem 1AA3 The Biology of Brainwashing Bees QMP contains homovanillyl alcohol (HVA)- HVA suppresses bad memories, but not good ones Biological role:- mitigates unpleasant side effects of QMP (?) HO HO NH 3 + dopamine O HO OH homovanillyl alcohol (HVA) 4 Chem 1AA3 The Biology of Brainwashing Bees Significance- HVA lowers [dopamine]- dopamine is associated with learning Therapeutic potential- high [dopamine] in some psychoses & schizophrenia- HVA-like molecules could be treatments BUT- need high specificity: attention deficit disorder & Parkinson's disease are linked to low [dopamine] global suppression of [dopamine], or suppression in the wrong parts of the brain, could have undesired side effects 5 Chem 1AA3 The Biology of Brainwashing Bees Key concepts queen bees control worker bees' memories using HVA help understanding human learning develop new therapeutics 6 Chem 1AA3 The Chemical Biology of Brainwashing Bees How do we go from a biological phenomenon to the development of a new therapeutic agent? 1 new drug requires ~5000 failed compounds improving synthesis & accelerating assays facilitates drug discovery 7 Chem 1AA3 High-throughput screening high-throughput screening (HTS): fast way to assay for a complex biological response 2 components: (1) a fast assay (2) massively parallel assays instead of doing experiments on live bees, use dopamine- producing bee neural cells growing in cell culture a fast, colour-producing dopamine assay already exists 8 Chem 1AA3 High-throughput screening 96 well microtiter plates allow 96 simultaneous assays grow bee neural cells in wells of plates test for dopamine production 9 Chem 1AA3 High-throughput screening some wells are used for standards and controls, others are for test compounds = high [dopamine] = no dopamine 10 10 Chem 1AA3 High-throughput screening screening must be able to test thousands of compounds per day, therefore it must be automated using robots and microtiter plates McMaster HTS Facility 11 11 Chem 1AA3 High-throughput screening = high [dopamine] = no dopamine grow neural cell cultures in 96 well plates, measure [dopamine] in response to test compounds 12 12 Chem 1AA3 High-throughput screening Key concepts high throughput assays that replicate complex biological phenomena require many compounds (more on this later) use microtiter plates highly automated 13 13...
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chem bio full note - Department of Department of 1...

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