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Unformatted text preview: plasmid can be distinguished on the basis of their resistance to that antibiotic. 4. A second selectable marker (that is, gene) which contains the polylinker and can be inactivated by insertion of the insert, thereby allowing a plasmid which contains an insert to be distinguished by the absence of the phenotype produced by that selectable marker. 5. Expression vectors have additional features (promoters) that allow high level expression of genes in the insert. 6. Shuttle vectors have an additional origin of replication to allow replication of the plasmid in a second type of host cell....
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This note was uploaded on 03/03/2010 for the course BIOL 214 taught by Professor Stark during the Spring '06 term at Illinois Tech.
- Spring '06