biology lab midterm 1 review - Chapter 1: Microscopy,...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Chapter 1: Microscopy, Cells, and Tissues -objective, ocular (eye piece), body tube, stage -compound microscope: single compound lens system (40x, 100x, and 400x), monoculars (oen eye piece) -dissecting microscope: 2 complete compound lens systems and has magnification from 8x to 50x (lacks components beneath the stage of the compound microscope Principles of Microscopy -light microscope: uses glass lenses and visible radiation: produce magnified image via light -stereoscopic microscope (dissecting): illuminated from above the stage -large distance between objective and object alls enough illumination from above so that the objective can collect light reflected by the object to form an image -compound: distance so small that the object is illuminated from below the stage -light first passes through the sub-stage condenser: a lens that focuses light on the object -microscope uses light transmitted by the object, the material must be transparent -image inverted -minimal resolvable distance: minimum distance between 2 point objects that can be distinguished in their images MRD= .6 lamda = .6 lamda N(sinx) numerical aperature -numerical aperature: nsinx -x=half the acceptance angle of the objective -total magnification product of magnifying powers of the objective and ocular Major Methods of Electron Microscopy -transimission: transmission of e- through object…2D…projector lens: lens in TEM that corresponds to the ocular…requires thin sections…usually stained -scanning: electron gun and condenser lens system to produced a finely primary e- beam -no lenses on other side of specimen…electrons emitted when struck, some are reflected by specimen’s surface…necessary to coat surface with a metal to enhance emission Using the Compound Microscope 1) clean ocular, objectives, substage with lens paper…place suvstage at highest position…rotate nosepiece so (40x) is above stage, rase stage until it is 3 mm below objective, turn on lamp low, remove the ocular lens and close ir is diaphram until diamter of illuminated circle is about ¾ its initial diameter … 2) looked at CORNELL’
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
-parfocal objectives: -fine focus knob: -working distance: distance between objective and object
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/03/2010 for the course BIOG 1101 taught by Professor N/a during the Fall '10 term at Cornell University (Engineering School).

Page1 / 5

biology lab midterm 1 review - Chapter 1: Microscopy,...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online