Genetics Lab - Objective: The pKa of an unknown indicator...

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Objective: The pKa of an unknown indicator will be determined by a pH meter and a visible spectrophometer. -The students must estimate pKa, determine the wave length of maximum absorbance for HIn and In-, and measure pKa. Trial 1 -The students obtained 50 mL of HCl and NaOH, 2 containers of water and added 5 drops of indicator into both adding HCl into one of them and NaOH into the second. Table 1: Observations of Colors and pH Observations HCl NaOH color yellow magenta pH 3.43 7.25 -The students added 10 mL NaOH to the container with HCl and added HCl drop-wise until the color changed. -The Number of Drops: around 300 drops -The students decided to take a different plan of action. Trial 2 -The students added 5 drops of acid in water with the indicator Observations: The solution turned yellow. -The students added 3 drops of NaOH Observations: The solution turned orange with a pH of 4.89. -10 mL HCl was put in a container with 1 drop of bromothymol blue, and the students used a spectrometer. Absorbance Peaks: 430 nm and 615 nm
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Genetics Lab - Objective: The pKa of an unknown indicator...

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