5.Rep_Lect09

5.Rep_Lect09 - Additional Reading Waga S, Stillman B. The...

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Additional Reading Waga S, Stillman B. The DNA replication fork in eukaryotic cells. Annu Rev Biochem. 1998;67:721-51. Some useful websites with annimations: http://www.mcb.harvard.edu/Losick/images/TromboneFINALd.s http://www.hhmi.org/biointeractive/animations/index.html
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The Meselon-Stahl Experiment-Semiconsevative Replication
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Autoradiogram and its interpretive drawing of a replicating E. coli chromosome. Page 1137
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Replication of DNA. Page 1137
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Autoradiographic differentiation of unidirectional and bidirectional θ replication of DNA. Page 1138
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Semidiscontinuous DNA replication. In DNA replication, both daughter strands ( leading strand red , lagging strand blue ) are synthesized in their 5 3 directions. Page 1138 Helicase unwinds DNA Topoisomerase removes supercoils
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Electron micrograph of a replication eye in Drosophila melanogaster DNA. Page 1139
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Priming of DNA synthesis by short RNA segments. Okazaki fragments were found to contain RNA at their 5’ end.
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Unwinding of DNA by the combined action of DnaB and SSB proteins. Page 1147 QuickTime™ and a TIF  (Uncompres ed) decompres or are ne ded to se  this picture.
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The replication of E. coli DNA. Page 1155
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Figure 30-15 Electron microscopy–based image reconstruction of T7 gene 4 helicase/primase. Page 1147
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Figure 30-16 X-Ray structure of the helicase domain of T7 gene 4 helicase/primase. Page 1147
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Figure 30-18 X-Ray structure of Rep helicase in complex with dT(pT) 15 and ADP. Page 1148
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Figure 30-17 Active, rolling mechanism for DNA unwinding by Rep helicase. Page 1148
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Figure 30-8a X-Ray structure of E. coli DNA polymerase I Klenow fragment (KF) in complex with a dsDNA. Page 1141
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Figure 30-9a X-Ray structure of Klentaq1 in complex with DNA and ddCTP. ( a ) The closed conformation. Page 1142
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Figure 30-9b X-Ray structure of Klentaq1 in complex with DNA. ( b ) The open conformation. Page 1142
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Figure 30-10 Schematic diagram for the nucleotidyl transferase mechanism of DNA polymerases. Page 1143
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X-Ray structure of the β subunit of E. coli Pol III holoenzyme. Ribbon drawing. Page 1146
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X-Ray structure of PCNA. Its three subunits ( red, green, and yellow ) form a 3-fold symmetric ring. DNA model drawn in. Page 1164
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The β subunit of E. coli Pol III holoenzyme. Space- filling model of sliding clamp in hypothetical complex with B-DNA. Page 1146
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QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. The Clamp Loader Kuriyan Cell Press 2001,
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The Clamp Loader QuickTime™ and a  decompressor are needed to see this picture. Figure 1. Structure of the Clamp Loader:DNA Complex (A) Schematic diagram of the clamp loader cycle. (B) Unbiased electron density for the DNA, calculated using a model at a stage prior to the inclusion of DNA and improved using density modification ( Terwilliger, 2000 ). Contour lines at 1.2 SD above the mean are shown in blue. The phosphate groups in the final DNA model are shown as spheres. The DNA-interacting
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5.Rep_Lect09 - Additional Reading Waga S, Stillman B. The...

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