200 2010 Midterm

200 2010 Midterm - Name:_ Biosci200/104 MIDTERM Winter,...

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Name:____________________________ Biosci200/104 MIDTERM Winter, 2010 - Before you start your exam, please write down your name on every page. - This is an open book, open note, open section paper exam, but use of the INTERNET is PROHIBITED! There is no time limit but the exam must be completed by 5 pm on Feb. 1 st . - Many questions can be answered a few sentences. Please be brief! -The exam must be your work alone. Talking to or working with anyone about any aspect of the exam is not allowed. The Stanford University Honor Code stipulates that students will not give or receive aid during an examination. I acknowledge and accept the Honor Code. Signature The exam due by 5 pm on Monday February 1 st , 2010 to Gilbert 208A. If you wish to turn in the exam at an earlier point, there will be a Drop Box to your exam in the administrative area of Dr. Gozani's office (208A Gilbert Hall) or you can turn it into one of the TAs. TEST has 100 points total. Bonus questions do not need to be answered and can increase your score only up to 100 pts.
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Name:____________________________ 1) Briefly explain the following: a) Why is the Pribnow box (a sequence of TATAATs in a promoter) an ideal place for unwinding? (2 pt) b) Why does the RNA polymerase slow down through G-C rich regions? (2 pt) 2) We discussed several aspects of the control of gene expression (the process that underlies the central dogma of molecular biology: DNA>RNA>Protein). Briefly describe (2 sentences) why the regulation of transcription is considered to be one of the most important regulatory steps in this process. (3 pts) 3) You have discovered a new gene called YFG that contributes to DNA repair efficiency. You notice that cells carrying YFG mutations frequently stop dividing and become senescent, and patients with the mutation suffer from premature ageing. Do you think that mutations in the YFG gene are likely to cause cancer? In no more than three sentences, please explain why you think YFG mutations might, or might not, lead to cancer (3 pts). 4) You are attempting to complete your N.E.W.T. (Nastily Exhausting Wizarding Test) in molecular magic. You believe you have identified the mysterious Parselmouth (PM) gene, which controls a wizard’s ability to talk to snakes. You have isolated PM cDNA sequence and you would like to do a number of molecular manipulations to understand how the protein functions.
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Name:____________________________ A. You would like to amplify the PM cDNA. However, at Hogwarts, you cannot buy enzymes from Muggle companies. Thus, you use a spell to bring thermophilic bacteria to your lab and to isolate their DNA polymerase. Briefly explain why you need a DNA polymerase enzyme from a thermophilic organism to perform PCR? (2 pts) B. You amplify the PM cDNA using PCR and the thermophilic DNA polymerase and then sequence your PM cDNA to make sure it is ok. You find that there is a mutation that seems to have come from the PCR reaction. Based on the amount of cDNA amplified during the PCR reaction, you calculate that the polymerization was due to an error rate of ~ 1X10 5 . Addition of what type of factor to the reaction will increase fidelity of your PCR reaction? (2 pts) C.
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200 2010 Midterm - Name:_ Biosci200/104 MIDTERM Winter,...

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