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ProblemSet5 - BIS103-001 Spring 08 Study Question Set 5 for...

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BIS103-001 Spring 08 Study Question Set 5 for Midterm 2 - 1 - Study Questions Set 5- Electron Transport Chain, Oxidative Phosphorylation 1. Several lines of evidence have been cited for the arrangement of components in the ETC. Cite two of these lines of evidence. What is meant by the phrase "respiration is tightly coupled to phosphorylation in intact mitochondria"? 2. The following series of electron carriers were isolated from Antarctic aardvark heart mitochondria. They are believed to be involved in the complete oxidation of reduced substrates. Predict which electron transfer reactions are sufficiently exergonic to "drive" ATP synthesis. Assume an "efficiency" of 50%. F = 96.5 kJ/Vmol. Standard State Electron Carrier Reduction Potential ( E o ') NAD - 0.32 v Flavoprotein - 0.29 v Quinone + 0.07 v Non-heme iron protein + 0.25 v Cytochrome d (fictitous) + 0.29 v Cytochrome e (fictitious) + 0.45 v O 2 +0.82 v 3. Define the term "uncoupler" as it is applied to the electron transport system. Name two uncouplers and their mechanisms of action.
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