Jan 13 (1)

Jan 13 (1) - Well start off with Isoelectric Focusing from...

Info iconThis preview shows pages 1–13. Sign up to view the full content.

View Full Document Right Arrow Icon
We’ll start off with Isoelectric Focusing from Last Day
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Making Antibodies to Look into Cells & as Therapeutic Reagents to Fight Disease "A microscopist thinks with the eyes and sees with the brain." Prof. Daniel Mazia, 1996
Background image of page 2
Monoclonal Antibodies Pages 400-402 Fig. 9-35 Cesar Milstein Nobel Prize 1984 www.sumanasinc.com
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
How Do We Use the Antibody? You are responsible for reading part on Western (immunoblotting) technology (pg. 98-99)
Background image of page 4
How are images obtained? FIG. 9-10 Barrier Filter Hg Lamp
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
- dyes or FLUOROPHORES absorb energy kicking electrons (e - ) into a higher orbital (unstable) - instability causes e - to drop into its normal orbital releasing energy as visible light “FLUORESCENCE” How is Image Seen?
Background image of page 6
- visible & UV light travel to ocular but only visible light is seen since barrier filter blocks harmful UV - signals, are bright on a black background
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Laser Scanning Confocal Microscope Exceptional clarity 3D reconstruction 4D imaging
Background image of page 8
Image Acquisition Fig. 9-18
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
ELECTRON MICROSCOPY pages 388-391 Figure 9-14
Background image of page 10
All Shapes & Sizes
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Fig. 9-20 Types of EM’s 3D / Surface detail
Background image of page 12
Image of page 13
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/04/2010 for the course SCI 2290 taught by Professor Kraj,dean,z during the Spring '10 term at UWO.

Page1 / 26

Jan 13 (1) - Well start off with Isoelectric Focusing from...

This preview shows document pages 1 - 13. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online