Jan 27

Jan 27 - MITOSIS & CELL CYCLE CONTROL pages 781-783,...

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MITOSIS & CELL CYCLE CONTROL pages 781-783, Chapter 20 Fig. 20-11
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Concept: -cell proliferation depends on signals -progression through cell cycle is regulated -check points ensure things should proceed: Is all DNA replicated? Is cell big enough? Is environment favorable? Is DNA damaged?
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Yet Another Nobel Story -involves 3 protein families, 2 of which are enzymes i) Kinase s ii) Phosphatase s iii) Cyclins Hartwell, Hunt & Nurse, 2001 yeast
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Regulation of Cell Cycle Yeast Mutations & Phenotypes Modified from Fig. 20-4 complementation Cdc2 ts Cdc2 Transformed Cdc2 ts Transform with plasmid library of wild-type S. Pombe DNA Cdc2 Mutated S. pombe I am going to use Cdc2 in place of 28 FUNCTIONAL COMPLEMENTATION
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CDC’s: The players: - mutating cdc2 gene prevents S.pombe from entering G 2 -M -cdc2 is transcribed & translated throughout cell cycle -cdc2 encodes a protein kinase (p34 cdc2 ) in all eukaryotes Fig. 20-11 20-12
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-cyclin is encoded by cdc13 gene -p34 cdc2 : cyclin dependent kinase (cdk) -p34 cdc2 + cyclin make MPF (mitosis promoting factor) -p34 cdc2 in MPF is inactive for most of cell cycle p34 activity p34 amount G1 S G2 M Player 2
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Jan 27 - MITOSIS & CELL CYCLE CONTROL pages 781-783,...

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