Bis2C.Lecture9.final

Bis2C.Lecture9.final - Transmission of traits(Genes genomes...

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Transmission of traits (Genes, genomes, and gene transfer) Bis 2C Lecture 9 October 10, 2008
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Reminder on Reading • Main reading: Chapter 26 • Other reading (mostly short) Chapter 1.2: How is all life related Chapter 4.2: Characteristics of prokaryotic cells Chapter 13.3: Gene exchange Chapter 13.5: Genomes Rothschild extremophile paper
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Outline I. More on uncultured II. Transmission of traits
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I. Uncultured
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Great Plate Count Anomaly Solution Culturing Microscope Count Count <<<< Solution?
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How Study Microbes Without Culturing? • Classification? (Or “Who is out there?”) • Studying their insides? (Or, “What are they doing?”)
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Great Plate Count Anomaly Solution Culturing Microscope Count Count <<<< DNA
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11.23 The Polymerase Chain Reaction
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Figure 8B - RNA Alignment
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rRNA Phylotyping • Extract DNA • Run rDNA PCR • Sequence products • Infer evolutionary tree
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rRNA and Uncultured Microbes Eisen et al. 1992
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Censored Censored
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Bis2C.Lecture9.final - Transmission of traits(Genes genomes...

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