Cell Biology Fall 2004 Exam 1

Cell Biology Fall 2004 Exam 1 - Principles of Cell Biology...

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Principles of Cell Biology Exam 1 Fall 2004 October 7, 2004 Directions: There are 75 questions in this exam with only one correct answer. All answers are to be placed on the grid sheet. Good luck! 1. Which of the following types of mutations identifies redundant pathways? a. Missense mutations b. Synthetic lethality mutation-2 c. Synthetic lethality mutation-1 d. Suppressor mutations e. None of the above 2. You are presented with cells in culture and you need to determine the amount of collagen being secreted into the medium. Which of the following techniques would be best for attacking this problem? a. Complementation analysis b. Cloning (genomic, not mRNA type) c. ELISA d. FRET e. In situ hybridization 3. SDS or sodium dodecyl sulfate has a molecular structure/characteristic most closely similar to which of the following? a. Proteins b. Phospholipids c. DNA cut as a consequence of restriction nucleases (like EcoR1) d. Carbohydrates such as sucrose, glucose e. ATP/GTP 4. Critical micelle concentration (CMC) discussed in your text is critical to appreciate when performing which of the following processes? a. Tracking protein synthesis b. Tracking DNA synthesis c. Analyzing complementation processes in yeast, but not bacteria d. Solubilizing membranes e. Accomplishing cDNA microarray analysis 5. Botulinum Toxin can be used for both good and bad purposes relying on the same mechanism of action. What does botox do to cells? a. It blocks the release of acetylcholine from neurons b. It interferes with dopamine synthesis c. It directly blocks the release of calcium in the sarcoplasmic reticulum of muscle d. It acts like RNAi e. It blocks the synthesis of neuropeptides within the axon terminal 6. In situ hybridization is a technique that relies on which of the following? a. Nucleic acid hybridization b. Microscopy c. Autoradiographic, colorimetric, fluorescent or chemiluminescent probes d. Intracellular injection e. All of the above
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7. Which type of cell produces monoclonal antibodies? a. A cells b. B cells c. T cells d. Red blood cells e. None of the above 8. Fluo3-AM, Rhodamine-123 and Calcein-AM are all probes used in what type of analysis? a. Ultrastructural immunocytochemistry b. FRET c. Examining physiological activities of living cells d. Labeling DNA on a Southern Blot e. Labeling RNA on a Northern Blot 9. cDNA microarrays are often used to analyze a. protein conformation b. gene expression c. relative sizes of genomes d. mitochondrial function e. all of the above 10. Autoradiography is a technique used for microscopy, biochemistry (protein separation) and molecular biology. What does it do? a. It detects radioactive probes. b. It qantifies fluorescence in cells using bottom-reading spectrofluorometers such as the CytoFluor c. It is the final process in SELDI-TOF immediately after the time of flight laser analysis of proteins flying off the substrate d. It is an automated system that processes cells and tissues for bright field microscopy e. It is the process that is used to cut ultrathin sections for transmission electron
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This note was uploaded on 03/07/2010 for the course BIOL 12311 taught by Professor Van during the Spring '10 term at SUNY Brockport.

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Cell Biology Fall 2004 Exam 1 - Principles of Cell Biology...

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