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YinProblemSet1 - glucose and lactose note on the curve...

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Name: GDCB 511 Problem Set 1, Jan 23, due Jan 28, 2009 Answer the following questions in 2-3 pages. Typing is recommended except for models, which should be drawn by hand . Write clearly if you have to. 1. (7 points) What are the main methods used to study protein:DNA and protein :protein interactions in vitro and/or in vivo. Describe the method briefly (3-4 sentences for each), discuss the applications and at least one advantage and one disadvantage for each method. 2. (6 points): Draw a growth curve of bacteria growing in media that contain both
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Unformatted text preview: glucose and lactose, note on the curve what’s happening at each stage. Draw molecular models and explain how do lactose and glucose control Lac gene expression? 3. (7 points) How does E. coli sense the level of tryptophan (Trp) to control its biosynthesis (consider both operon and attenuation mechanisms)? Draw models to facilitate your explanation. Design a new Trp sensor in the attenuation mechanism that DOES NOT involve the TrpL (leader); explain how your sensor would work....
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