YinProblemSet2Key

YinProblemSet2Key - Name: Genetics 511 Problem Set 2, due...

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Name: Genetics 511 Problem Set 2, due on Friday Feb 20, 2009 1. (12 points) (a) Draw a model of the eukaryotic basic transcription machinery on a typical RNA polymerase II core promoter (2 points); (b) Briefly describe the functions of TFIIB, TFIID (TBP and TAFs), TFIIE, TFIIF and TFIIH (6 points). (c) How do transcription activators promote transcription (you don’t have to consider chromatin here, 4 points). (a) See lecture notes for model (b) TFIID: TBP (TATA-box binding protein): bind TATA box, recognizes promoter and bends DNA to fit to the RNA polymerase to start transcription initiation. TAFs: -recruits TBP to TATA-less promoters -targets for different activators TFIIB: linker (bridge) between TFIID and RNA polymerase II/TFIIF TFIIF: binds/recruits RNA polymerase II and interacts with non-template DNA TFIIH: -phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II, which is required for transition from transcription initiation to elongation -causes full melting at the promoter by its helicase activity
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This note was uploaded on 03/08/2010 for the course GDCB 511 taught by Professor Yanhaiyin during the Spring '09 term at Iowa State.

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YinProblemSet2Key - Name: Genetics 511 Problem Set 2, due...

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