{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

GDCB511Exam2-09Key

GDCB511Exam2-09Key - GDCB 511 Exam II 2009 100 Points Name...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
GDCB 511 Exam II 2009 Name: 100 Points 1. (10 points): The RNA polymerase II (Pol II) structure is shown below. BRIEFLY describe the functions of (2 points each): Cleft: to accept DNA template Active site: at the bottom of the cleft to synthesize RNA Clamp: maintain transcription bubble with several structures Rbp4/7 may regulate clamp in close/open states Bridge helix (not shown in the structure): facilitate transcription translocation QuickTimeª and a TIFF (Uncompressed) decompressor are needed to see this picture.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
2. (15 points): This is a GMSA experiment with a DNA probe containing TATA box and general transcription factors and/or RNA polymerase II (Pol II). From the experiment, what can you conclude about the functions of TFIID, TFIIB and TFIIF in the assembly of the transcription initiation complex (12 points)? Draw a model to facilitate your explanation (3 points). (a) TFIID: bind TATA box, required for the assembly of the initiation complex (lane 13) (b) TFFIIB: bridge between TFIID and Pol II (lane 14, lane 2) (c) TFIIF: recruit Pol II to DB complex, required for assembly of preinitiation complex (lanes 8-12) 2
Image of page 2
3. (10 pints): List two of TFIIH’s enzyme activities and their functions.
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern