GDCB511Exam2-09Key

GDCB511Exam2-09Key - GDCB 511 Exam II 2009 100 Points Name:...

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GDCB 511 Exam II 2009 Name: 100 Points 1. (10 points): The RNA polymerase II (Pol II) structure is shown below. BRIEFLY describe the functions of (2 points each): Enhanceosome Mediator complex Cleft: to accept DNA template Active site: at the bottom of the cleft to synthesize RNA Clamp: maintain transcription bubble with several structures Rbp4/7 may regulate clamp in close/open states Bridge helix (not shown in the structure): facilitate transcription translocation QuickTimeª and a TIFF (Uncompressed) decompressor are needed to see this picture.
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2. (15 points): This is a GMSA experiment with a DNA probe containing TATA box and general transcription factors and/or RNA polymerase II (Pol II). From the experiment, what can you conclude about the functions of TFIID, TFIIB and TFIIF in the assembly of the transcription initiation complex (12 points)? Draw a model to facilitate your explanation (3 points). (a) TFIID: bind TATA box, required for the assembly of the initiation complex (lane 13) (b) TFFIIB: bridge between TFIID and Pol II (lane 14, lane 2) (c) TFIIF: recruit Pol II to DB complex, required for assembly of preinitiation complex (lanes 8-12) 2
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3. (10 pints): List two of TFIIH’s enzyme activities and their functions.
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This note was uploaded on 03/08/2010 for the course GDCB 511 taught by Professor Yanhaiyin during the Spring '09 term at Iowa State.

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GDCB511Exam2-09Key - GDCB 511 Exam II 2009 100 Points Name:...

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