GDCB 511-4-22-09

GDCB 511-4-22-09 - GDCB 511: 22 April 2009 New Homework...

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GDCB 511: 22 April 2009 New Homework Assignment Lecture today: DNA Damage and Repair Homologous Recombination Weaver Chapter 22, 718 – 740 RecA RecBCD
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Fig. 20.41
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Fig. 20.42
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Fig. 20.43
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Fig. 20.44 Error-prone and error-free bypass in Humans Bypass DNA polymerases: ξ , η , θ , ι , κ
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Summary of DNA repair mechanisms Repair mechanism Enzymes involved in repair O6-methylguanine methyl transferase Removal of base alkylation Photoreactivation Photolyase Base excision repair ( E. coli ) DNA glycosylase, AP endonuclease, DNA phosphodiesterase, DNA pol I, DNA ligase Base excision repair (humans) glycosylase, APE1, DNA pol β, DNA ligase Nucleotide excision repair (E. coli) UvrABC endonuclease, DNA pol I, DNA ligase Nucleotide excision repair (humans) XPC-hHR23B (RNA pol in the case of transcription-coupled NER), XPA, RPA, TFIIH, ERCC1-XPF & XPG endonucleases, DNA polymerase, DNA ligase
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Summary of DNA repair mechanisms (continued) Repair mechanism Enzymes involved in repair MutH, MutL, MutS, exonuclease I, helicase, DNA polymerase III, SSB, DNA ligase, methyl transferase Mismatch repair in E. coli Non-homologous end-joining Ku, DNA-PK CS , DNA ligase Homologous repair (covered extensively in the next chapter)
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Homologous Recombination in E. coli : the RecBCD pathway Recombination begins at the site of a DNA break RecBCD binds to the blunt end and cuts at the 3’ end of a Chi site (GCTGGTGG). Chi sites found on average every 5000 bp in
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GDCB 511-4-22-09 - GDCB 511: 22 April 2009 New Homework...

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