GDCB 511 5_1_09 REVIEW

GDCB 511 5_1_09 REVIEW - GDCB 511, May 1, 2009 Exam IV &...

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GDCB 511, May 1, 2009 Exam IV & Final Review Announcements Both Exam 4 and the Final will be held at 7:30— 9:30 AM, Tuesday, May 5, Atanasoff Hall B29 ,y , y , :30- :30, Exam 4=100 points 7:30 8:30, Exam 4 100 points 8:30-9:30, Final=100 points (50 +50)
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Overview of splicing reactions
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verview of snRNA associations Overview of snRNA associations U1 base pairs with the 5’ splice site U2 base pairs with the conserved sequence at the splicing branchpoint 4 base pairs with U6 U4 base pairs with U6 U6 base pairs with the 5’ splice site; U6 also base airs with U2 pairs with U2 U5 associates with the last nucleotide in one exon and the first nucleotide of the next, presumably lining up the exons for splicing
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Compare cis-splicing (left) Vs. ans plicing elow) trans-splicing (below).
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What is the mechanism of NA editing? RNA editing? . Nuclease detects mis- 1. Nuclease detects mis match and cuts pre-mRNA at site of non-base pairing. 2. The mismatched “U” residue in the pre-mRNA can be excised by a 3’ RNA exonuclease; or, a “U” residue can be added by TUTase (terminal uridylyl transferase) 3. The cut is resealed by RNA ligase. Editing proceeds from 3’ to 5’ until the entire mRNA is edited.
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Figure 15.26 Factors involved in 3’ end formation and polyadenylation CPSF: Cleavage and polyadenylation specificity factor at binds AAUAAA signal; that binds AAUAAA signal; CstF: Cleavage stimulation factor that binds downstream G/U-rich region; CF I/ CF II: Cleavage factors that cut RNA transcript; PolyA polymerase (PAP): atalyzes polyA synthesis; catalyzes polyA synthesis; PolyA Binding protein II (PABII): Binds growing polyA tail and stimulates polyA addition.
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• Cleavage in mammals requires several proteins – CPSF – cleavage and polyadenylation specificity factor – CstF – cleavage stimulation factor – CF I – Cleavage factor I – CF II – Cleavage factor II – Poly (A) polymerase – RNA polymerase II
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iRNAs are derived lin-4, let-7 C. elegans miRNAs are derived from primary transcripts (pri-miRNAs) that form tem op stem-loop structures with the miRNA in a base paired animal pre-miRNAs plant pre-miRNAs region. Most miRNA genes are located in a different part of genome than their target mRNA. But some are encoded in an intron of their target RNA. mRNA.
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Fig. 16.45 MicroRNAs regulate gene expression. p First discovered in C. elegans , microRNAs are now known to be important regulators of gene expression in animals and plants. They act by blocking translation (animals) or triggering the RNAi pathway (plants).
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echanisms of chromosomal DNA replication Mechanisms of chromosomal DNA replication in bacteria and bacteriophage Basic Features: 1. Template copied via complementary basepairing roposed by Watson &Crick (proposed by Watson &Crick, 1953) emi onservative: 2. Semi-Conservative: Meselson and Stahl, 1958.
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This note was uploaded on 03/08/2010 for the course GDCB 511 taught by Professor Yanhaiyin during the Spring '09 term at Iowa State.

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GDCB 511 5_1_09 REVIEW - GDCB 511, May 1, 2009 Exam IV &...

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