GDCB 511 Homework_YANG_4-13-09

GDCB 511 Homework_YANG_4-13-09 - 1 GDCB 511 Homework...

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GDCB 511: Homework Assignment Due 4/20/2009: “When Polymerases Collide” Homework assignment is based on the following paper: Liu et al., 1993. The DNA replication fork can pass RNA polymerase without displacing the nascent transcript. Nature 366, 33-39. A PDF of the paper is posted on WebCT. Answer the questions in italics regarding Figure 1 and Figure 2. Figure 1. Critique this experiment. Do you see any potential flaw in this experiment or the conclusions? How could the experiment be improved? Figure 2. Critique this experiment. Do you see any potential flaw in this experiment or the conclusions? How could the experiment be improved? Background to the paper: How do DNA replication and RNA transcription co-exist? Both RNA polymerase and DNA polymerase have to separate the helices and synthesize new polynucleotide strands based upon complementary basepairing. Replication is 10x faster than transcription; in Drosophila, transcription of Ubx (Ultrabithorax) takes longer than the time of a cell cycle. Are RNA polymerase and transcripts “bumped off” the DNA by the replication fork?
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This note was uploaded on 03/08/2010 for the course GDCB 511 taught by Professor Yanhaiyin during the Spring '09 term at Iowa State.

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GDCB 511 Homework_YANG_4-13-09 - 1 GDCB 511 Homework...

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