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Metnet_lamdaphage_cooperativity

Metnet_lamdaphage_cooperativity - Lecture Outline •...

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Unformatted text preview: Lecture Outline • Information and Examples of Cooperativity • Introduction to the Lamdaphage System • Modeling the lamdaphage • Bistability • Reading and Sources: • Notes and paper by Hasty Cooperativity • If protein has several binding sites, then interactions get more complex – Positive cooperativity: can make binding easier for the substrate – Negative: decreases affinity of protein to other ligands Positive Homotrophic cooperativity • Protein with multiple identical binding sites • Look at dimerized protein, E 2 . 2 2 2 2 2 slow fast E S E S E S S E S + → + → Overall rate • n is the number of binding sites max max 1 n B n B V K S v V Y K S = = + n=1 n=3 n=2 Multiple Binding Sites • Proteins bind multiple substrates • Assume protein has n binding sites for a substrate • One step in reaction: 1 j j k j j k S P P-- + 1 j j j P K P S- = 1 2 1 2 1 2 1 n n k k k n n k k k S P P P P P---- + L 1 0 1 1 1 2 1 2 2 1 0 1 1 2 1 2 2 dP k P S k P dt dP k PS k P k P S k P dt k PS k P---- = - + = = - + +- = - + = 1 j j j j j k P K k P S-- = = General Rate Equation...
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Metnet_lamdaphage_cooperativity - Lecture Outline •...

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