Metnet_lamdaphage_cooperativity

Metnet_lamdaphage_cooperativity - Lecture Outline...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Lecture Outline Information and Examples of Cooperativity Introduction to the Lamdaphage System Modeling the lamdaphage Bistability Reading and Sources: Notes and paper by Hasty Cooperativity If protein has several binding sites, then interactions get more complex Positive cooperativity: can make binding easier for the substrate Negative: decreases affinity of protein to other ligands Positive Homotrophic cooperativity Protein with multiple identical binding sites Look at dimerized protein, E 2 . 2 2 2 2 2 slow fast E S E S E S S E S + + Overall rate n is the number of binding sites max max 1 n B n B V K S v V Y K S = = + n=1 n=3 n=2 Multiple Binding Sites Proteins bind multiple substrates Assume protein has n binding sites for a substrate One step in reaction: 1 j j k j j k S P P-- + 1 j j j P K P S- = 1 2 1 2 1 2 1 n n k k k n n k k k S P P P P P---- + L 1 0 1 1 1 2 1 2 2 1 0 1 1 2 1 2 2 dP k P S k P dt dP k PS k P k P S k P dt k PS k P---- = - + = = - + +- = - + = 1 j j j j j k P K k P S-- = = General Rate Equation...
View Full Document

Page1 / 33

Metnet_lamdaphage_cooperativity - Lecture Outline...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online