Gel Electrophoresis theory

Gel Electrophoresis theory - 12/30/2007 1 Gel...

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Unformatted text preview: 12/30/2007 1 Gel Electrophoresis Electrophoresis Defn: A separation technique that uses electricity to move charged molecules through a matrix Arne Tiselius c 1937. Invented moving boundary electrophoresis to separate proteins based on charge c 1948. Awarded the Nobel Prize in Chemistry for his invention. Moving boundary electrophoresis The big idea Before electrophoresis After electrophoresis + ++ ++ ++ + + Positive pole +++++++++++++++++----------------- Negative pole--- + ++ ++ ++ + + Positive pole +++++++++++++++++----------------- Negative pole--- The moving boundary cell a.k.a. Tiselius cell 2 Problems with the Tiselius cell c Cant get the molecules out of the cell without mixing them. c Incomplete separation: Many proteins have the same net charge. 12/30/2007 2 Solutions c Stabilize movement of molecules by using semi-solid MATRICES instead of fluid c 1950?- Paper c 1955- Starch c 1957- Cellulose acetate (TLC) c 1959- Polyacrylamide c Late 1970s- Agarose Agarose...
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This note was uploaded on 03/08/2010 for the course BIOL 100L taught by Professor Claassen during the Spring '08 term at UMBC.

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Gel Electrophoresis theory - 12/30/2007 1 Gel...

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