Measuring Liquids

Measuring Liquids - 12/30/2007 Types of liquid transfer...

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12/30/2007 1 Measuring Liquids Types of liquid transfer devices Serological Pipette Transfer pipettes Pasteur Disposable Graduated Cylinders Micropipettor Choosing a liquid transfer device Considerations • Precision • Capacity When moving liquids is the goal (Precision not important) Volume > 2 ml Volume 0.1-2 ml Beaker Transfer Pipettes Disposable or Pasteur pipettes?? Easier to use (bulb attached) Comes pre- sterilized (irradiation) Less convenient (bulb separate) Must autoclave to sterilize Pros Cons degrades Irradiation generates nuclear waste Pros Cons Eco-friendly NEVER When precision is important 10-2,000 ml 0.1-10 ml 0.1 to 0.001 ml (1-100 μl) Match volume to capacity
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12/30/2007 2 USING PIPETTES • Rules for all pipettes • How to use transfer pipettes • How to use serological pipettes • How to use micropipettors USING PIPETTES These rules are true, no matter what type of pipette you use: • Don’t touch the tips. • Once filled, keep pipettes in vertical, tip-down
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This note was uploaded on 03/08/2010 for the course BIOL 100L taught by Professor Claassen during the Spring '08 term at UMBC.

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Measuring Liquids - 12/30/2007 Types of liquid transfer...

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